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Western Blot at 1:1000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Jurkat cell line lysates (35ug/lane).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.

LGALS4 Antibody

CATALOG NUMBER: 56-392

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This LGALS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human LGALS4.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LGALS4
Additional Names:
Galectin-4, Gal-4, Antigen NY-CO-27, L-36 lactose-binding protein, L36LBP, Lactose-binding lectin 4, LGALS4
Protein Accession Number:
P56470
Protein Gi Number:
3024079
Ncbi Gene Id Number:
3960
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The galectins are a family of beta-galactoside-bindingproteins implicated in modulating cell-cell and cell-matrixinteractions. The expression of this gene is restricted to smallintestine, colon, and rectum, and it is underexpressed incolorectal cancer.
Background Reference 1:
Zimbardi, A.L., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 66 (PT 5), 542-545 (2010) :
Background Reference 2:
Stechly, L., et al. Traffic 10(4):438-450(2009)
Background Reference 3:
Tripodi, D., et al. BMC Med Genomics 2, 65 (2009) :
Background Reference 4:
Oberg, C.T., et al. Bioorg. Med. Chem. Lett. 18(13):3691-3694(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-392

List Size:
400 ul

List Price:

$495.00

Datasheet
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