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Western blot analysis of extracts of various cell lines, using LGALS8 antibody (22-376) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using LGALS8 antibody (22-376) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using LGALS8 antibody (22-376) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using LGALS8 antibody (22-376). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using LGALS8 antibody (22-376) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

LGALS8 Antibody

CATALOG NUMBER: 22-376

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-317 of human LGALS8 (NP_963838.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
BT-474
Positive Control 2:
22Rv1
Positive Control 3:
Mouse liver
Positive Control 4:
Mouse small intestine
Predicted Molecular Weight:
Observed: 38kDa/50kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LGALS8
Additional Names:
LGALS8, Gal-8, PCTA-1, PCTA1, Po66-CBP
Ncbi Gene Id Number:
3964
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-376

List Size:
50 uL

List Price:

$285.00

Out of stock

Datasheet
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