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Western blot analysis of extracts of various cell lines, using LGR5 antibody (13-711) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of NIH/3T3 cells using LGR5 antibody (13-711) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using LGR5 antibody (13-711) at dilution of 1:100.
Western blot analysis of extracts of various cell lines, using LGR5 antibody (13-711) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

LGR5 Antibody

CATALOG NUMBER: 13-711

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 824-907 of human LGR5 (NP_003658.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:1000 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
HT-29
Positive Control 2:
HepG2
Positive Control 3:
SKOV3
Positive Control 4:
A-431
Positive Control 5:
Mouse brain
Positive Control 6:
Rat brain
Predicted Molecular Weight:
Observed: 105kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LGR5
Additional Names:
Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67
Ncbi Gene Id Number:
8549
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-711

List Size:
100 uL

List Price:

$436.00

Datasheet
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