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Human LGR5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human R-Spondin 3 at 2 ug/mL (100 uL/well) can bind Human LGR5, His Tag with a linear range of 5-39 ng/mL (QC tested).
Human LGR5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

LGR5 / GPR49 Recombinant Protein

CATALOG NUMBER: 11-137

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Gly 22 - Arg 561
Fusion Tag:
His Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 62.0 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted Molecular Weight:
62.0 kDa
Purity:
>90% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
LGR5
Additional Names:
LGR5, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, Leucine-rich repeat-containing G-protein coupled receptor 5
Protein Accession Number:
NP_003658.1
Ncbi Gene Id Number:
8549
Background:
LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.
Background Reference 1:
Morgan RG. et al. Br J Cancer. 2018 May 29; 118(11): 1410–1418.
Background Reference 2:
Yue Z. et al. FASEB J. 2018 May;32(5):2422-2437.
Background Reference 3:
de Lau W. et al. Genes & development. 2014;28:305–16.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-137

List Size:
0.1 mg

List Price:

$577.00

Datasheet
Shipping Info

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