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Western blot analysis of LIAR in mouse kidney tissue lysate with LIAR antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of LIAR in mouse kidney tissue with LIAR antibody at 2.5 μg/mL.
Immunofluorescence of LIAR in Mouse Kidney cells with LIAR antibody at 20 μg/mL.
Western blot analysis of LIAR in mouse kidney tissue lysate with LIAR antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

LIAR Antibody

CATALOG NUMBER: 5261

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
LIAR antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human LIAR.
The immunogen is located within amino acids 230 - 280 of LIAR.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
LIAR antibody can be used for detection of LIAR by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Purification:
LIAR Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LIAR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LIAR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ANKRD54
Additional Names:
LIAR Antibody: LIAR, LIAR, Ankyrin repeat domain-containing protein 54, Lyn-interacting ankyrin repeat protein
Protein Accession Number:
NP_620152
Protein Gi Number:
20270347
Ncbi Gene Id Number:
129138
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LIAR Antibody: Ankyrin (ANK) repeats mediate protein-protein interactions in diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20. ANK repeats may occur in combinations with other types of domains. The structural repeat unit contains two anti-parallel helices and a beta-hairpin, with repeats stacked in a superhelical arrangement. LIAR, also known as ANKRD54, is a recently identified ANK repeat-containing protein that is predominantly expressed in tissues rich in cilliated cells, such as olfactory sensory neurons and is predicted to be important to cilia. At least three isoforms of LIAR are known to exist.
Background Reference 1:
Li J, Mahajan A, and Tsai MD. Ankyrin repeat: a unique motif mediating protein-protein interactions. Biochemistry2006; 45:15168-78.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5261

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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