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LIF Recombinant Protein

CATALOG NUMBER: 91-014

Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
Ser23-Phe202
Fusion Tag:
Tag Free
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Predicted Molecular Weight:
19.7 kD
Biological Activity:
ED50 is less than 0.01 ng/ml as determined by the M1 cell differentiation assay.
Specific Activity of 1.0 x 108 IU/mg.
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 0.02% Tween 20, pH 7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Storage Conditions:
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at -20°C for 3 months.
Ncbi Official Symbol:
LIF
Additional Names:
Leukemia Inhibitory Factor, LIF, Differentiation-Stimulating Factor, D Factor, Melanoma-Derived LPL Inhibitor, MLPLI, Emfilermin, HILDA
Protein Accession Number:
P15018
Protein Gi Number:
20149322
Ncbi Gene Id Number:
3976
Background:
Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

91-014

List Size:
0.05 mg

List Price:

$515.00

Datasheet
Shipping Info

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