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Western blot analysis in CEM cell line lysates (35ug/lane).
Western blot analysis in CEM cell line lysates (35ug/lane).

LIG3 Antibody

CATALOG NUMBER: 58-255

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This LIG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 793-822 amino acids from the C-terminal region of human LIG3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
113 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LIG3
Additional Names:
DNA ligase 3, DNA ligase III, Polydeoxyribonucleotide synthase [ATP] 3, LIG3
Protein Accession Number:
P49916
Protein Gi Number:
251757259
Ncbi Gene Id Number:
3980
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the DNA ligase family. Eachmember of this family encodes a protein that catalyzes the joiningof DNA ends but they each have a distinct role in DNA metabolism.The protein encoded by this gene is involved in excision repair andis located in both the mitochondria and nucleus, with translationinitiation from the upstream start codon allowing for transport tothe mitochondria and translation initiation from a downstream startcodon allowing for transport to the nucleus. Additionally,alternate transcriptional splice variants, encoding differentisoforms, have been characterized.
Background Reference 1:
Wang, W., et al. Nucleic Acids Res. (2010) In press :
Background Reference 2:
Arora, M., et al. Leukemia 24(8):1470-1475(2010)
Background Reference 3:
Cotner-Gohara, E., et al. Biochemistry 49(29):6165-6176(2010)
Background Reference 4:
Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-255

List Size:
400 ul

List Price:

$495.00

Datasheet
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