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Antibody used in IHC on Human Pancreas.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in IHC on Human Pancreas.

LIG4 Antibody

CATALOG NUMBER: 28-008

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LIG4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
LIG4 antibody can be used for detection of LIG4 by ELISA at 1:312500. LIG4 antibody can be used for detection of LIG4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
104 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store LIG4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
LIG4
Additional Names:
LIG4,
Protein Accession Number:
NP_002303
Protein Gi Number:
23199993
Ncbi Gene Id Number:
3981
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V (D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.
Background Reference 1:
Smith, J., et al., (2003) Nucleic Acids Res. 31 (8), 2157-2167.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-008

List Size:
100 ul

List Price:

$473.00

Datasheet
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