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Human LILRB2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
Human LILRB2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

LILRB2 Recombinant Protein

CATALOG NUMBER: 96-515

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Gln 22 - Val 461
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
48.6 kDa
Biological Activity:
Measured by its ability of the immobilized protein to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 80000 cells/well are added to LILRB2 coated plates (8. 8μg/mL with 100μL/well), > 40% cells will adhere for 1 hour incubation at 37°C.
Purity:
>92% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
LILRB2
Additional Names:
LILRB2, ILT4, LIR2, MIR10, CD85d
Protein Accession Number:
AAH36827
Ncbi Gene Id Number:
10288
Background:
Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Background Reference 1:
Borges L., et al., 1997, J. Immunol. 159:5192-5196.
Background Reference 2:
Fanger N.A., et al., 1998, Eur. J. Immunol. 28:3423-3434.
Background Reference 3:
Shiroishi M., et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 100:8856-8861.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-515

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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