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Western blot analysis of LIMA1 in human colon tissue lysate with LIMA1 antibody at (A) 0.5 and (B) 1 μg/ml.
Immunohistochemistry of LIMA1 in human colon tissue with LIMA1 antibody at 5 μg/mL.
Immunofluorescence of LIMA1 in human colon tissue with LIMA1 antibody at 20 μg/mL.
Western blot analysis of LIMA1 in human colon tissue lysate with LIMA1 antibody at (A) 0.5 and (B) 1 μg/ml.
Trial Size

LIMA1 Antibody

CATALOG NUMBER: 8053

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (89%), Pig: (72%)
Immunogen:
LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1.
The immunogen is located within amino acids 230 - 280 of LIMA1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist; this antibody will only detect the three largest isoforms.
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Predicted Molecular Weight:
Predicted: 50, 66, 84 kDa
Observed: 65 kDa
Purification:
LIMA1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LIMA1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LIMA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
LIMA1
Additional Names:
LIM domain and actin-binding protein 1, PP624, EPLIN, SREBP3
Protein Accession Number:
NP_001107018
Protein Gi Number:
165905589
Ncbi Gene Id Number:
51474
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).
Background Reference 1:
Maul RS and Chang DD. EPLIN, epithelial protein lost in neoplasm. Oncogene 1999; 18:7838-41.
Background Reference 2:
Maul RS, Song Y, Amann KJ, et al. EPLIN regulates actin dynamics by cross-linking and stabilizing filaments. J. Cell Biol. 2003; 160:399-407.
Background Reference 3:
Abe K and Takeichi M. EPLIN mediates linkage of the cadherin catenin complex to F-actin and stabilizes the circumferential actin belt. Proc. Natl. Acad. Sci. USA 2008; 105:13-9.
Background Reference 4:
Zhang S, Wang X, Iqval S, et al. Epidermal growth factor promotes protein degradation of epithelial protein lost in neoplasm (EPLIN), a putative metastasis suppressor, during epithelial-mesenchymal transition. J. Biol. Chem. 2013; 288:1469-79.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8053

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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