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Western blot analysis in mouse brain tissue lysates (35ug/lane).
LIMD1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse brain tissue lysates (35ug/lane).

LIMD1 Antibody

CATALOG NUMBER: 56-870

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This LIMD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-467 amino acids from the C-terminal region of human LIMD1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
72 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LIMD1
Additional Names:
LIM domain-containing protein 1, LIMD1
Protein Accession Number:
Q9UGP4
Protein Gi Number:
47605932
Ncbi Gene Id Number:
8994
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LIMD1 is a transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.
Background Reference 1:
Ghosh, S., et al. Mol. Cancer 9, 58 (2010) :
Background Reference 2:
Sharp, T.V., et al. Proc. Natl. Acad. Sci. U.S.A. 105(50):19932-19937(2008)
Background Reference 3:
Spendlove, I., et al. Int. J. Cancer 123(10):2247-2253(2008)
Background Reference 4:
Huggins, C.J., et al. Cancer Lett. 267(1):55-66(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-870

List Size:
400 ul

List Price:

$495.00

Datasheet
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