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Western blot analysis of lysed extracts from UV-treated C2C12, PMA-treated HeLa and anisomycin-treated 293 cells using LIMK1 (Phospho-Thr508).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 (Phospho-Thr508).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using LIMK1 (Phospho-Thr508).
Western blot analysis of lysed extracts from UV-treated C2C12, PMA-treated HeLa and anisomycin-treated 293 cells using LIMK1 (Phospho-Thr508).

LIMK1 (phospho Thr508) Antibody

CATALOG NUMBER: 79-158

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
LIMK1 (Phospho-Thr508) antibody was raised against a peptide sequence around phosphorylation site of threonine 508 (R-Y-T (p) -V-V) derived from Human LIMK1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of LIMK1 only when phosphorylated at threonine 508.
Predicted Molecular Weight:
72 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
LIMK1
Additional Names:
LIMK, LIMK-1, C1orf215, LAP18, OP18, kinase LIMK1
Protein Accession Number:
NP_002305.1
Protein Gi Number:
4505001
Ncbi Gene Id Number:
3984
Background:
Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.
Background Reference 1:
Soosairajah J, et al. (2005) EMBO J. Ohashi K, et al. (2000) J Biol Chem; 275 (5): 3577-82. Edwards DC, et al. (1999) Nat Cell Biol; 1 (5): 253-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-158

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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