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Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using LIMK2 (Phospho-Thr505).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK2 (Phospho-Thr505).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using LIMK2 (Phospho-Thr505).
Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using LIMK2 (Phospho-Thr505).

LIMK2 (phospho Thr505) Antibody

CATALOG NUMBER: 79-159

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
LIMK2 (Phospho-Thr505) antibody was raised against a peptide sequence around phosphorylation site of threonine 505 (R-Y-T (p) -V-V) derived from Human LIMK2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of LIMK2 only when phosphorylated at threonine 505.
Predicted Molecular Weight:
70 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
LIMK2
Additional Names:
PKB, RAC, LIMK-2, kinase LIMK2
Protein Accession Number:
NP_001026971.1
Protein Gi Number:
73390140
Ncbi Gene Id Number:
3985
Background:
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Background Reference 1:
Soosairajah J, et al. (2005) EMBO J. Ohashi K, et al. (2000) J Biol Chem; 275 (5): 3577-82. Edwards DC, et al. (1999) Nat Cell Biol; 1 (5): 253-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-159

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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