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Western blot analysis of lysed extracts from HepG2 cells using Lin28 Antibody.
Western blot analysis of lysed extracts from HepG2 cells using Lin28 Antibody.

Lin28 Antibody

CATALOG NUMBER: 79-647

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Lin28 antibody was raised against a peptide sequence around aa. 1~5 (M-G-S-V-S) derived from Human Lin28 .
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:1000
Specificity:
This antibody detects endogenous levels of total Lin28 protein.
Predicted Molecular Weight:
26 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
LIN28A
Additional Names:
CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A, CSDD1, LIN28A
Protein Accession Number:
NP_078950.1
Protein Gi Number:
13375938
Ncbi Gene Id Number:
79727
Background:
Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells.
Background Reference 1:
Heo I., Joo C., Mol. et al., Cell 32:276-284 (2008) Heo I., Joo C., Kim Y. -K., et al., Cell 138:696-708 (2009) Viswanathan S. R., Powers J. T., et al., Nat. Genet. 41:843-848 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-647

List Size:
0.1 mg

List Price:

$370.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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