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Western blot analysis of lysed extracts from HeLa and 293 cells using LIN28B Antibody.
Western blot analysis of lysed extracts from HeLa and 293 cells using LIN28B Antibody.

LIN28B Antibody

CATALOG NUMBER: 79-728

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
LIN28B antibody was raised against a peptide sequence around aa.242~246 ( P-S-V-Q-K) derived from Human LIN28B.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous levels of total LIN28B protein.
Predicted Molecular Weight:
21 32 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
LIN28B
Additional Names:
CSDD2, CTNNB, OK/SW-cl.35, PRO2286, CSDD2, FLJ16517
Protein Accession Number:
NP_001004317.1
Protein Gi Number:
51972214
Ncbi Gene Id Number:
389421
Background:
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Background Reference 1:
"Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma. "Guo Y., Chen Y., Ito H., et al. Gene 384:51-61 (2006) "Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA. "Heo I., Joo C., Cho J., Ha M., Han J., Kim V. N. Mol. Cell 32:276-284 (2008) "TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation. "Heo I., Joo C., Kim Y. -K., Kim V. N., et al. Cell 138:696-708 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-728

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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