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Western blot analysis of extracts of various cell lines, using LINS1 antibody (15-782) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using LINS1 antibody (15-782) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

LINS1 Antibody

CATALOG NUMBER: 15-782

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse LINS1 (NP_690028.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
Rat spleen
Positive Control 2:
Mouse testis
Positive Control 3:
Mouse spleen
Positive Control 4:
Rat testis
Predicted Molecular Weight:
Observed: 86kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LINS1
Additional Names:
2700083B01Rik, AW547219, Lins, Lins2, Wins2, protein Lines homolog 1, Wins2 protein with Drosophila Lin homologous domain, lines homolog 2, protein Lines homolog 2, protein with Drosophila Lines homologous domain, wnt-signaling molecule Lines homolog 1, wnt-signaling molecule Lines homolog 2
Ncbi Gene Id Number:
72635
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Drosophila segment polarity gene lin encodes a protein, lines, which plays important roles in development of the epidermis and hindgut. This gene encodes a protein containing a lines-like domain. This gene is located on chromosome 15 and clustered with the gene encoding ankyrin repeat and SOCS box-containing protein 7. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-782

List Size:
100 uL

List Price:

$436.00

Datasheet
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