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45-838 (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-838 (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-838 (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Lipocalin 2 Antibody

CATALOG NUMBER: 45-838

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-AILREDKDPQKMY
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 22kDa band observed in Human Kidney lysates (calculated MW of 22.6kDa according to NP_005555.2). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Spleen shows staining of cytoplasm in mostly binucleated cells, and of nucleoli in some of the non-dividing cells. Recommended concentration: 2-3ug/ml.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
LCN2
Additional Names:
LCN2, NGAL, lipocalin 2 (oncogene 24p3), HGNC:6526, uterocalin, oncogenic lipocalin 24p3, neutrophil gelatinase-associated lipocalin, lipocalin 2, MSFI, OTTHUMP00000022240, migration-stimulating factor inhibitor, neutrophil gelatinase-associated lipocalin, oncogene 24p3, siderocalin
Protein Accession Number:
NP_005555.2
Protein Gi Number:
38455402
Ncbi Gene Id Number:
3934
Background Reference 1:
Mishra J, Dent C, Tarabishi R, Mitsnefes MM, Ma Q, Kelly C, Ruff SM, Zahedi K, Shao M, Bean J, Mori K, Barasch J, Devarajan P. Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery. Lancet. 2005 Apr;365(9466):1231-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-838

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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