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Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.

LIPT1 Antibody

CATALOG NUMBER: 30-449

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LIPT1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
LIPT1 antibody can be used for detection of LIPT1 by ELISA at 1:312500. LIPT1 antibody can be used for detection of LIPT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
42 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store LIPT1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
LIPT1
Additional Names:
LIPT1, MGC12290, MGC13378,
Protein Accession Number:
NP_057013
Protein Gi Number:
7706252
Ncbi Gene Id Number:
51601
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The process of transferring lipoic acid to proteins is a two-step process. The first step is the activation of lipoic acid by lipoate-activating enzyme to form lipoyl-AMP. For the second step, LIPT1 transfers the lipoyl moiety to apoproteins.The process of transferring lipoic acid to proteins is a two-step process. The first step is the activation of lipoic acid by lipoate-activating enzyme to form lipoyl-AMP. For the second step, the protein encoded by this gene transfers the lipoyl moiety to apoproteins. Alternative splicing in the 5' UTR of this gene results in five transcript variants that encode the same protein.
Background Reference 1:
Fujiwara, K., (1999) Eur. J. Biochem. 260 (3), 761-767.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-449

List Size:
100 ul

List Price:

$519.00

Datasheet
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