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45-841 staining (1ug/ml) of Human Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
45-841 (10ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-841 staining (1ug/ml) of Human Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

LIRF Antibody

CATALOG NUMBER: 45-841

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig
Immunogen:
The immunogen for this antibody is: CDPRAPLRIVPAES
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40kDa band observed in Human Brain lysates (predicted MWs of 41kDa and 48kDa according to NP_079512.1 and NP_739575.1). Recommended for use at 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognize 2 of the 3 reported isoforms (NP_079512.1 and NP_739575.1 but not NP_739576.1)
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RNF39
Additional Names:
RNF39, LIRF, ring finger protein 39, HZFW1, HZFw1 protein, LTP (long-term potentiation) induced RING finger protein, DADB-60O11.8, HZF, HZFW
Protein Accession Number:
NP_079512.1,NP_739575.1
Protein Gi Number:
13376844
Ncbi Gene Id Number:
80352
Background Reference 1:
Matsuo R, Asada A, Fujitani K, Inokuchi K. LIRF, a gene induced during hippocampal long-term potentiation as an immediate-early gene, encodes a novel RING finger protein. Biochem Biophys Res Commun. 2001 Nov 30;289(2):479-84.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-841

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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