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Western blot analysis of LMBRD1 in human brain tissue lysate with LMBRD1 antibody at 1 μg/mL.
Immunohistochemistry of LMBRD1 in human brain tissue with LMBRD1 antibody at 2.5 μg/mL.
Immunofluorescence of LMBRD1 in human brain tissue with LMBRD1 antibody at 20 μg/mL.
Western blot analysis of LMBRD1 in human brain tissue lysate with LMBRD1 antibody at 1 μg/mL.
Trial Size

LMBRD1 Antibody

CATALOG NUMBER: 6047

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
LMBRD1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human LMBRD1.
The immunogen is located within the last 50 amino acids of LMBRD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
LMBRD1 antibody can be used for detection of LMBRD1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
LMBRD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LMBRD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LMBRD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LMBRD1
Additional Names:
LMBRD1 Antibody: NESI, LMBD1, MAHCF, C6orf209, NESI, BM-021, CD001, MSTP044, Probable lysosomal cobalamin transporter, HDAg-L-interacting protein NESI
Protein Accession Number:
NP_060838
Protein Gi Number:
261878497
Ncbi Gene Id Number:
55788
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LMBRD1 Antibody: LMBRD1, also known as NESI (nuclear export signal-interacting protein, is a lysosomal membrane protein that is thought be involved in the transport and metabolism of cobalamin. LMBRD1 was initially identified as interacting with the large form of the hepatitis delta antigen and may be required for the nucleocytoplasmic shuttling of the hepatitis delta virus. Mutations in this gene are associated with the vitamin B12 metabolism disorder termed, homocystinuria-megaloblastic anemia complementation type F (cblF).
Background Reference 1:
Gailus S, Hohne W, Gasnier B, et al. Insights into lysosomal cobalamin trafficking: lessons learned from cblF disease. J. Mol. Med.2010; 88:459-66.
Background Reference 2:
Wang Y-S, Chang SC, Huang C, et al. Novel nuclear export signal-interacting protein, NESI, critical for the assembly of hepatitis delta virus. J. Virol.2005; 79:8113-20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6047

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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