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45-843 staining (1ug/ml) of Human Lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
45-843 staining (1ug/ml) of Human Lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

LMO7 Antibody

CATALOG NUMBER: 45-843

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: ESTCVSASLPRS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70-75kDa band and approx 30kDa band observed in Human Lung lysates (predicted MWs of 92kDa according to AAD33924.1 - however our result agrees with Putilina et al as below). A minor band of unknown identity was also consistently observed at approx 50kDa. Recommended for use at 0.5-2ug/ml.
Specificity:
Please note that the amino acid sequence of the immuning peptide differs one amino acid from the current NCBI accession numbers (NP_005349.3, NP_056667.2). This antibody is expected to recognise both reported isoforms (NP_005349.3; NP_056667.2).
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
LMO7
Additional Names:
LMO7, FBXO20, LOMP, FBX20, KIAA0858, LIM domain only 7, LOMP protein, F-box protein Fbx20, F-box only protein 20, zinc-finger domain-containing protein, RP11-332E3.2
Protein Accession Number:
AAD33924.1
Protein Gi Number:
4929268
Ncbi Gene Id Number:
4008
Background Reference 1:
Putilina T, Jaworski C, Gentleman S, McDonald B, Kadiri M, Wong P. Analysis of a human cDNA containing a tissue-specific alternatively spliced LIM domain. Biochem Biophys Res Commun. 1998 Nov 18;252(2):433-9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-843

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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