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<strong>Figure 1 Immunofluorescence Validation of LMX1A in HeLa Cells</strong><br>Immunofluorescent analysis of methanol-fixed HeLa cells labeling LMX1A with 7087 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
<strong>Figure 2 Western Blot Validation in Human Skeletal Muscle </strong><br>Loading: 10 μg of lysates per lane.Aμg ntibodies: LMX1A, 7087, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse Skeletal Muscle </strong><br>Loading: 10 μg of lysates per lane.Antibodies: LMX1A, 7087, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Rat Skeletal Muscle </strong><br>Loading: 10 μg of lysates per lane.Antibodies: LMX1A, 7087, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunohistochemistry Validation of LMX1A in Human Brain Meningioma</strong><br> Immunohistochemical analysis of paraffin-embedded human brain meningioma using anti-LMX1A antibody (7087) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4#730;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunohistochemistry Validation of LMX1A in Mouse Brain Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- anti-LMX1A antibody (7087) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4#730;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of LMX1A in Rat Skeletal Muscle Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-LMX1A antibody (7087) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 Immunofluorescence Validation of LMX1A in HeLa Cells
Immunofluorescent analysis of methanol-fixed HeLa cells labeling LMX1A with 7087 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Trial Size

LMX1A Antibody

CATALOG NUMBER: 7087

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB, ICC/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (92%)
Immunogen:
Rabbit polyclonal LMX1A antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LMX1A.
The immunogen is located within amino acids 300 - 350 of LMX1A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB, ICC/IF
Application Note:
WB: 1-2 μg/mL; ICC/IF: 10-20 μg/mL; IHC-P: 1-5 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested.
Specificity:
At least two isoforms are known to exist. This antibody is predicted to not cross-react with LMX1B.
Positive Control 1:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Positive Control 2:
Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate
Positive Control 3:
Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate
Predicted Molecular Weight:
Predicted: 42kD

Observed: 45 kD
Isoforms:
Human LMX1A has two isoforms, including isoform 1 (382aa, 42.7kD) and isoform 2 (133aa, 14.6kD). Mouse LMX1A one isoform (382aa, 42.9kD), and ratt LMX1A has one isoform (382aa, 42.8kD). 7087 can detect human, mouse and rat.
Purification:
LMX1A Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LMX1A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LMX1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LMX1A
Additional Names:
LMX1A Antibody: LMX1, LMX1.1, LIM homeobox transcription factor 1-alpha
Protein Accession Number:
NP_796372
Protein Gi Number:
28893581
Ncbi Gene Id Number:
4009
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LMX1A Antibody: The LIM homeobox transcription factor 1 (LMX1A) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1A binds an A/T-rich sequence and functions in the nucleus as a transcriptional activator to the insulin gene promoter. In the developing embryo, LMX1A is expressed along the neuraxis and leads to the development of the roof plate of the vertebrae. While LMX1A is crucial for the differentiation of dopamine neurons, the related LMX1B appears to be important for both the development and the survival of these cells. It has been suggested that LMX1A might be a useful biomarker or risk factor in tumor aggressiveness.
Background Reference 1:
Failli et al. Mech. Dev. 2002; 118:225-8.
Background Reference 2:
Nakatani et al. Biol. 2010; 339:101-13.
Background Reference 3:
Chizhikov and Millen. Development 2004; 131:2693-705.
Background Reference 4:
Lin et al. Am. J. Clin. Pathol. 2012; 137:971-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7087

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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