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Western blot analysis of LNPEP in human lung tissue lysate with LNPEP antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of LNPEP in human lung tissue with LNPEP antibody at 20 μg/mL.
Western blot analysis of LNPEP in human lung tissue lysate with LNPEP antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

LNPEP Antibody

CATALOG NUMBER: 7055

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (73%), Rat: (73%)
Immunogen:
LNPEP antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human LNPEP.
The immunogen is located within amino acids 900 - 950 of LNPEP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
LNPEP antibody can be used for detection of LNPEP by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, LNPEP often migrates at 165kD in SDS-PAGE.
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
113 kDa
Purification:
LNPEP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LNPEP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LNPEP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LNPEP
Additional Names:
LNPEP Antibody: CAP, IRAP, PLAP, P-LAP, OTASE, Leucyl-cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Cystinyl aminopeptidase
Protein Accession Number:
NP_005566
Protein Gi Number:
61742777
Ncbi Gene Id Number:
4012
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LNPEP Antibody: LNPEP, also known as insulin-responsive aminopeptidase (IRAP), was initially identified as an abundant protein that is associated with specialized endosomes termed GLUT4 storage vesicles (GSVs) in adipocytes. The luminal domain of LNPEP contains an aminopeptidase activity that is involved in peptide hormone processing and antigenic peptide processing. LNPEP is also a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to the GSVs.
Background Reference 1:
Keller SR, Scott HM, Mastick CC, et al. Cloning and characterization of a novel insulin-regulated membrane aminopeptidase from Glut4 vesicles. J. Biol. Chem. 1995; 270:23612-8.
Background Reference 2:
Wallis MG, Lankford MF, and Keller SR. Vasopressin is a physiological substrate for the insulin-regulated aminopeptidase IRAP. Am. J. Physiol. Endocrinol. Metab. 2007; 293:E1092-102.
Background Reference 3:
Saveanu L, Carroll O, Weimershaus M, et al. IRAP identifies an endosomal compartment required for MHC class I cross-presentation. Science 2009; 325:213-7.
Background Reference 4:
Jordens I, Molle D, Xiong W, et al. Insulin-regulated aminopeptidase is a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to specialized insulin-regulated vesicles. Mol. Biol. Cell 2010; 21:2034-44.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7055

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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