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Western Blot at 1:2000 dilution Lane 1: human lung lysate Lane 2: human spleen lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
LOXL3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:2000 dilution Lane 1: human lung lysate Lane 2: human spleen lysate Lysates/proteins at 20 ug per lane.

LOXL3 Antibody

CATALOG NUMBER: 56-683

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This LOXL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 715-744 amino acids from the C-terminal region of human LOXL3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
83 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LOXL3
Additional Names:
Lysyl oxidase homolog 3, 143-, Lysyl oxidase-like protein 3, LOXL3, LOXL
Protein Accession Number:
P58215
Protein Gi Number:
14916616
Ncbi Gene Id Number:
84695
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the lysyl oxidase genefamily. The prototypic member of the family is essential to thebiogenesis of connective tissue, encoding an extracellularcopper-dependent amine oxidase that catalyses the first step in theformation of crosslinks in collagens and elastin. A highlyconserved amino acid sequence at the C-terminus end appears to besufficient for amine oxidase activity, suggesting that each familymember may retain this function. The N-terminus is poorly conservedand may impart additional roles in developmental regulation,senescence, tumor suppression, cell growth control, and chemotaxisto each member of the family. Alternatively spliced transcriptvariants of this gene have been reported but their full-lengthnature has not been determined.
Background Reference 1:
Kim, Y., et al. Oncol. Rep. 22(4):799-804(2009)
Background Reference 2:
Sebban, S., et al. Virchows Arch. 454(1):71-79(2009)
Background Reference 3:
Akagawa, H., et al. Hum. Genet. 121 (3-4), 377-387 (2007) :
Background Reference 4:
Lee, J.E., et al. J. Biol. Chem. 281(49):37282-37290(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-683

List Size:
400 ul

List Price:

$495.00

Datasheet
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