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Western blot analysis of extracts of various cell lines, using LPCAT4 antibody (15-481) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using LPCAT4 antibody (15-481) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

LPCAT4 Antibody

CATALOG NUMBER: 15-481

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 345-524 of human LPCAT4 (NP_705841.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
HT-29
Positive Control 2:
HeLa
Positive Control 3:
HepG2
Positive Control 4:
Mouse brain
Positive Control 5:
Mouse testis
Predicted Molecular Weight:
Observed: 57kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LPCAT4
Additional Names:
AGPAT7, AYTL3, LPAAT-eta, LPEAT2, lysophospholipid acyltransferase LPCAT4, 1-AGP acyltransferase 7, 1-AGPAT 7, 1-acylglycerol-3-phosphate O-acyltransferase 7 (lysophosphatidic acid acyltransferase, eta), 1-acylglycerophosphocholine O-acyltransferase, 1-acylglycerophosphoserine O-acyltransferase, 1-alkenylglycerophosphoethanolamine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, PLSC domain containing protein, acyl-CoA:lysophosphatidylethanolamine acyltransferase 2, acyltransferase-like 3, lysophosphatidylethanolamine acyltransferase 2, plasmalogen synthase
Ncbi Gene Id Number:
254531
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-481

List Size:
100 uL

List Price:

$436.00

Datasheet
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