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Western blot analysis of LPIN1 in K562 cell lysate with LPIN1 antibody at 1 μg/mL.
Immunohistochemistry of LPIN1 in human small intestine tissue with LPIN1 antibody at 5 μg/ml.
Immunofluorescence of LPIN1 in human small intestine tissue with LPIN1 antibody at 20 μg/ml.
Western blot analysis of LPIN1 in K562 cell lysate with LPIN1 antibody at 1 μg/mL.
Trial Size

LPIN1 Antibody

CATALOG NUMBER: 7393

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
LPIN1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human LPIN1.
The immunogen is located within the last 50 amino acids of LPIN1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
LPIN1 antibody can be used for detection of LPIN1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
LPIN1 antibody is human and mouse reactive. At least four isoforms of LPIN1 are known to exist.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
Predicted: 107 kDa
Observed: 125 kDa
Purification:
LPIN1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LPIN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LPIN antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
LPIN1
Additional Names:
LPIN1 Antibody: PAP1, KIAA0188, Phosphatidate phosphatase LPIN1, Lipin-1
Protein Accession Number:
NP_001248357
Protein Gi Number:
387528013
Ncbi Gene Id Number:
23175
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LPIN1 Antibody: LPIN1, also known as PAP1, is a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol (reviewed in 1). LPIN1 is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism (1,2). Mutations in LPIN1 are associated with metabolic diseases such as type 2 diabetes and recurrent acute myoglobinuria (3,4) and it is also a candidate for several human lipodystrophy syndromes (5).
Background Reference 1:
Reue K and Zhang P. The lipin protein family: dual roles in lipid biosynthesis and gene expression. FEBS Lett. 2008; 582:90-6.
Background Reference 2:
Peterfy M, Phan J, Xu P, et al. Lipodystrophy in the fld mouse results from mutation of a new gene encoding a nuclear protein, lipin. Nat. Genet. 2001; 27:121-4.
Background Reference 3:
Zhang R, Jiang F, Hu C, et al. Genetic variants of LPIN1 indicate an association with Type 2 diabetes mellitus in a Chinese population. Diabet. Med. 2013; 30:118-22.
Background Reference 4:
Zeharia A, Shaag A, Houtkooper RH, et al. Mutations in LPIN1 cause recurrent acute myoglobinuria in childhood. Am. J. Hum. Genet. 2008; 83:489-94.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7393

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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