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Western blot analysis using LPP monoclonal antibody against truncated LPP - His recombinant protein and Hela cell lysate.
Western blot analysis using LPP monoclonal antibody against truncated LPP - His recombinant protein and Hela cell lysate.

LPP Antibody [8B3A11]

CATALOG NUMBER: 32-187

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Purified truncated recombinantLPP-His is expressed in E.Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IF:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
LPP monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LPP
Additional Names:
LPP protein
Protein Accession Number:
NP_001161143.1
Protein Gi Number:
264681524
Ncbi Gene Id Number:
4026
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LIM containing lipoma preferred partner, it belongs to the zyxin family. The Zyxin family of proteins contains five members, Ajuba, LIMD1, LPP, TRIP6 and Zyxin. LPP, an 80 kDa protein and a LIM domain-containing scaffolding protein contains three LIM domains at its carboxy terminus, which are preceded by a proline-rich pre-LIM region containing a number of protein interaction domains. The LPP localizes to sites of cell adhesion, such as focal adhesions and cell-cell contacts, and shuttles to the nucleus where it has transcriptional activation capacity.
Background Reference 1:
Mol Cell Proteomics. 2005 Sep;4(9):1240-50. Epub 2005 Jun 11.
Background Reference 2:
Cancer Genet Cytogenet. 2005 Nov;163(1):68-70.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-187

List Size:
0.1 mL

List Price:

$495.00

Datasheet
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