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Western blot analysis in Hela,Jurkat,NCI-H460 cell line lysates (35ug/lane).
LPPR4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela,Jurkat,NCI-H460 cell line lysates (35ug/lane).

LPPR4 Antibody

CATALOG NUMBER: 61-737

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This LPPR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 666-695 amino acids from the C-terminal region of human LPPR4.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
83 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LPPR4
Additional Names:
Lipid phosphate phosphatase-related protein type 4, Brain-specific phosphatidic acid phosphatase-like protein 1, Plasticity-related gene 1 protein, PRG-1, LPPR4, KIAA0455, PRG1
Protein Accession Number:
Q7Z2D5
Protein Gi Number:
74750018
Ncbi Gene Id Number:
9890
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LPPR4 belongs to the lipid phosphate phosphatase (LPP) family. LPPs catalyze the dephosphorylation of a number of bioactive lipid mediators that regulate a variety of cell functions. This protein is specifically expressed in neurons. It is located in the membranes of outgrowing axons and has been shown to be important for axonal outgrowth during development and regenerative sprouting.
Background Reference 1:
Trimbuch, T., et al. Cell 138(6):1222-1235(2009)
Background Reference 2:
Vasan, R.S., et al. JAMA 302(2):168-178(2009)
Background Reference 3:
Brauer, A.U., et al. Nat. Neurosci. 6(6):572-578(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-737

List Size:
400 ul

List Price:

$495.00

Datasheet
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