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<strong>Figure 1 WB Validation of LpxT in E. coli </strong><br>Loading: 15 μg of lysate Antibodies: LpxT 10051, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 WB Validation of LpxT in E. coli
Loading: 15 μg of lysate Antibodies: LpxT 10051, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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LpxT Antibody

CATALOG NUMBER: 10051

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
E. coli
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
E. coli
Homology:
Predicted species reactivity based on immunogen sequence: Shigella: (100%), Citrobacter freundii: (91%)
Immunogen:
Anti-LpxT antibody (10051) was raised against an 18 amino acid peptide near the carboxy terminus of E. coli LpxT.

The immunogen is located within the last 50 amino acids of LpxT.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 0.5 μg/mL.

Antibody validated: Western Blot in E.coli samples. All other applications and species not yet tested.
Predicted Molecular Weight:
Predicted: 27 kD

Observed: 27 kD
Purification:
LpxT Antibody is affinity chromatography.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LpxT Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LpxT Antibody can be stored at 4 ° C for three months and -20 ° C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
lpxT
Additional Names:
Lipid A 1-diphosphate synthase, Kdo(2)-lipid A phosphotransferase, Undecaprenyl pyrophosphate:lipid A 1-phosphate phosphotransferase, Eck2168, yeiU
Protein Accession Number:
P76445.2
Protein Gi Number:
3183418
Ncbi Gene Id Number:
946693
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LpxT is involved in the modification of the lipid A domain of lipopolysaccharides (LPS) and transfers a phosphate group from undecaprenyl pyrophosphate (C55-PP) to lipid A to form lipid A 1-diphosphate as well as contributes to the recycling of undecaprenyl phosphate (C55-P). In vitro, it has low undecaprenyl-diphosphate phosphatase activity.
Background Reference 1:
Touze, et al. Mol Microbiol. 2008; 67(2): 267-77.
Background Reference 2:
Tatar, et al. Microbiology 2007; 153(Pt8): 2518-2529.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

10051

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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