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Western blot analysis of LRFN4 in rat brain lysate with LRFN4 antibody at 1 μg/mL.
Immunohistochemistry of LRFN4 in mouse brain tissue with LRFN4 antibody at 2.5 μg/mL.
Immunofluorescence of LRFN4 in mouse brain tissue with LRFN4 antibody at 20 μg/mL.
Western blot analysis of LRFN4 in rat brain lysate with LRFN4 antibody at 1 μg/mL.
Trial Size

LRFN4 Antibody

CATALOG NUMBER: 5077

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
LRFN4 antibody was raised against a 14amino acid synthetic peptide near the carboxy terminus of human LRFN4.
The immunogen is located within amino acids 550 - 600 of LRFN4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
LRFN4 antibody can be used for detection of LRFN4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
This antibody is predicted to not cross-react with other members of the LRFN family.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
LRFN4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LRFN4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LRFN4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LRFN4
Additional Names:
LRFN4 Antibody: SALM3, SALM3., FIGLER6, SALM3, Leucine-rich repeat and fibronectin type-III domain-containing protein 4
Protein Accession Number:
Q6PJG9
Protein Gi Number:
62286924
Ncbi Gene Id Number:
78999
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LRFN4 Antibody: LRFN4 is one of a family of five transmembrane glycoproteins that are highly expressed in neuronal tissues. LRFN proteins share leucine-rich repeat (LRR)-immunoglobulin-like (Ig)-fibronectin type III (Fn)-transmembrane domain structure with other members of the LRR-Ig-Fn protein superfamily such as the Slitrk family of proteins. Expression of LRFN1, -3, and -4 mRNA was detected in embryonic neuronal cells, while Lrfn2 and Lrfn5 expression was primarily restricted to more mature cells. LRFN1, -2, and -4 bound to PDZ domains of postsynaptic PSD95, re-distributing PSD95 to the cell periphery. It has been suggested that the Lrfn proteins play a role in the developing and/or mature vertebrate nervous system. At least two isoforms of LRFN4 are known to exist.
Background Reference 1:
Morimura N, Inoue T, Katayama K, et al. Comparative analysis of structure, expression and PSD95-binding capacity of Lrfn, a novel family of neuronal transmembrane proteins. Gene2006; 380:72-83.
Background Reference 2:
Kobe B and Kajava AV. The leucine-rich repeat as a protein recognition motif. Curr. Opin. Struct. Biol.2001; 11:725-32.
Background Reference 3:
Aruga J and Mikoshiba K. Identification and characterization of Slitrk, a novel transmembrane protein family controlling neurite outgrowth. Mol. Cell Neurosci.2003; 24:117-29.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5077

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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