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Western blot analysis of extracts of various cell lines, using LRPAP1 antibody (18-902) at 1:200 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using LRPAP1 Antibody (18-902) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using LRPAP1 Antibody (18-902) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using LRPAP1 Antibody (18-902) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using LRPAP1 Polyclonal Antibody (18-902) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using LRPAP1 antibody (18-902) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

LRPAP1 Antibody

CATALOG NUMBER: 18-902

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human LRPAP1
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
K-562
Positive Control 2:
THP-1
Positive Control 3:
HepG2
Predicted Molecular Weight:
Observed: 39kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LRPAP1
Additional Names:
LRPAP1, A2MRAP, A2RAP, HBP44, MGC138272, MRAP, RAP, MYP23, alpha-2-MRAP
Ncbi Gene Id Number:
4043
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that interacts with the low density lipoprotein (LDL) receptor-related protein and facilitates its proper folding and localization by preventing the binding of ligands. Mutations in this gene have been identified in individuals with myopia 23. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-902

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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