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Western blot analysis of lysates from human blood plasma tissue (from left to right),using LRRC45 Antibody .AP11368b was diluted at 1:1000 at each lane.
Immunofluorescent analysis of U251 cells, using LRRC45 Antibody . Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
LRRC45 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from human blood plasma tissue (from left to right),using LRRC45 Antibody .AP11368b was diluted at 1:1000 at each lane.

LRRC45 Antibody

CATALOG NUMBER: 55-810

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This LRRC45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 615-643 amino acids from the C-terminal region of human LRRC45.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:25
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
76 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LRRC45
Additional Names:
Leucine-rich repeat-containing protein 45, LRRC45
Protein Accession Number:
Q96CN5
Protein Gi Number:
74760765
Ncbi Gene Id Number:
201255
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background Reference 1:
Olsen, J.V., et al. Cell 127(3):635-648(2006) Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-810

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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