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Western blot analysis of LRRC8A in EL4 cell lysate with LRRC8A antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of LRRC8A in rat spleen tissue with LRRC8A antibody at 2.5 μg/mL.
Western blot analysis of LRRC8A in EL4 cell lysate with LRRC8A antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

LRRC8A Antibody

CATALOG NUMBER: 8235

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
LRRC8A antibody was raised against a 15 amino acid peptide near the amino terminus of human LRRC8A.
The immunogen is located within amino acids 60 - 110 of LRRC8A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
LRRC8A antibody can be used for detection of LRRC8A by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Specificity:
LRRC8A antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Predicted Molecular Weight:
Predicted: 89 kDa
Observed: 95 kDa
Purification:
LRRC8A antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LRRC8A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LRRC8A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
LRRC8A
Additional Names:
Leucine rich repeat containing 8 family member A, leucine-rich repeat-containing protein 8A, LRRC8, AGM5
Protein Accession Number:
NP_062540
Protein Gi Number:
62241040
Ncbi Gene Id Number:
56262
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The leucine rich repeat containing 8 family member A protein (LRRC8A) belongs the LRRC8 family of proteins within the LRR superfamily of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions (1,2). LRRC8A is a four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation (1). LRRC8A has also been shown to be an essential component of the volume-regulated anion channel VRAC (3).
Background Reference 1:
Sawada A, Takihara Y, Kim JY, et al. A congenital mutation of the novel gene LRRC8 causes agammaglobulinemia in humans. J. Clin. Invest. 2003; 112:1707-13.
Background Reference 2:
Kubota K, Kim JY, Sawada A, et al. LRRC8 involved in B cell development belongs to a novel family of leucine-rich repeat proteins. FEBS Lett. 2004; 564:147-52.
Background Reference 3:
Voss FK, Ullrich F, Munch J, et al. Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC. Science 2014; 344:634-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8235

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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