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Western blot analysis of LRRFIP1 in human colon tissue lysate with LRRFIP1 antibody at 1 μg/mL.
Immunohistochemistry of LRRFIP1 in human colon tissue with LRRFIP1 antibody at 5 μg/mL.
Immunofluorescence of LRRFIP1 in human colon tissue with LRRFIP1 antibody at 20 μg/mL.
Western blot analysis of LRRFIP1 in human colon tissue lysate with LRRFIP1 antibody at 1 μg/mL.
Trial Size

LRRFIP1 Antibody

CATALOG NUMBER: 6219

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
LRRFIP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human LRRFIP1.
The immunogen is located within the last 50 amino acids of LRRFIP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
LRRFIP1 antibody can be used for detection of LRRFIP1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
LRRFIP1 antibody is predicted to not cross-react with other LRRFIP family members. Three isoforms of LRRFIP1 are known to exist; this antibody will only recognize the two longer isoforms.
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Purification:
LRRFIP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LRRFIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LRRFIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LRRFIP1
Additional Names:
LRRFIP1 Antibody: GCF2, TRIP, FLAP1, GCF-2, FLAP-1, HUFI-1, FLIIAP1, GCF2, Leucine-rich repeat flightless-interacting protein 1, GC-binding factor 2, LRR FLII-interacting protein 1
Protein Accession Number:
NP_001131024
Protein Gi Number:
212276096
Ncbi Gene Id Number:
9208
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LRRFIP1 Antibody: LRRFIP1, also known as GC-binding factor 2 (GCF2), is a 738 amino acid transcriptional repressor that mainly plays a cytoskeletal role. It is primarily localized in the cytoplasm and preferentially binds to GC-rich dsDNA, but will also bind directly to dsRNA as well. The RNA binding domain encompasses a lysine-rich motif. LRRFIP1 interacts with the mammalian Flightless I (Fli-I) and is a key component in the cytoskeletal regulation of platelet function. LRRFIP1 and the related protein LRRFIP2 may modulate canonical WNT signaling and mediate the IRF3-induced production of type I interferon via a beta-catenin-dependent pathway.
Background Reference 1:
Rikiyama T, Curtis J, Oikawa M, et al. GCF2: expression and molecular analysis of repression. Biochim. Biophys. Acta 2003; 1629:15-25.
Background Reference 2:
Suriano AR, Sanford AN, Kim N, et al. GCF2/LRRFIP1 represses tumor necrosis factor α expression. Mol. Cell. Biol. 2005; 25:9073-81.
Background Reference 3:
Wilson SA, Brown EC, Kingsman AJ, et al. TRIP: a novel double-stranded RNA binding protein which interacts with the leucine-rich repeat of Flightless I. Nucleic Acids Res. 1998; 26:3460-7.
Background Reference 4:
Goodall AH, Burns P, Salles I, et al. Transcription profiling in human platelets reveals LRRFIP1 as a novel protein regulating platelet function. Blood 2010; 116:4646-56.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6219

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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