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Western blot analysis of extracts of various cell lines, using LRRK2 antibody (13-870) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using LRRK2 antibody (13-870) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

LRRK2 Antibody

CATALOG NUMBER: 13-870

Clonality:
Polyclonal
Tested Applications:
Flow, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide of human LRRK2
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:20 - 1:50
Flow: 1:20 - 1:50
Positive Control 1:
LO2
Positive Control 2:
A-549
Positive Control 3:
293T
Positive Control 4:
Jurkat
Predicted Molecular Weight:
Observed: 286kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LRRK2
Additional Names:
LRRK2, PARK8, ROCO2, FLJ45829, DKFZp434H2111, leucine-rich repeat kinase 2, dardarin, Parkinson disease (autosomal dominant) 8
Ncbi Gene Id Number:
120892
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-870

List Size:
100 uL

List Price:

$365.00

Out of stock

Datasheet
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