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Western blot analysis of LRRTM1 in mouse brain tissue lysate with LRRTM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of LRRTM1 in human brain tissue with LRRTM1 antibody at 5 μg/mL.
Immunofluorescence of LRRTM1 in human brain tissue with LRRTM1 antibody at 20 μg/mL.
Western blot analysis of LRRTM1 in mouse brain tissue lysate with LRRTM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

LRRTM1 Antibody

CATALOG NUMBER: 6167

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
LRRTM1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human LRRTM1.
The immunogen is located within amino acids 450 - 500 of LRRTM1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
LRRTM1 antibody can be used for detection of LRRTM1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
LRRTM1 antibody is predicted to not cross-react with other LRRTM family members.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
LRRTM1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LRRTM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LRRTM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LRRTM1
Additional Names:
LRRTM1 Antibody: Leucine-rich repeat transmembrane neuronal protein 1
Protein Accession Number:
AAH45113
Protein Gi Number:
86990456
Ncbi Gene Id Number:
347730
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LRRTM1 Antibody: The Leucine-rich repeat transmembrane neuronal proteins (LRRTMs) are differentially expressed in the nervous system and were recently found to instruct presynaptic and mediate postsynaptic glutamatergic differentiation, with LRRTM1 and LRRTM2 most potent at inducing presynaptic differentiation. Each LRRTM protein is a type I transmembrane containing ten extracellular leucine-rich repeats and a short intracellular tail and has a developmentally regulated pattern distinct from all others. LRRTM1 is a maternally suppressed gene that is associated paternally with handedness and schizophrenia.
Background Reference 1:
Lauren J, Airaksinen MS, Saarma M, et al. A novel gene family encoding leucine-rich repeat transmembrane protein differentially expressed in the nervous system. Genomics 2003; 81:411-21.
Background Reference 2:
Linhoff MW, Lauren J, Cassidy RM, et al. An unbiased expression screen for synaptogenic proteins identifies the LRRTM protein family as synaptic organizers. Neuron 2009; 61:734-49.
Background Reference 3:
Siddiqui TJ, Pancaroglu R, Kang Y, et al. LRRTMs and neuroligins bind neurexins with a differential code to cooperate in glutamate synapse development. J. Neurosci. 2010; 30:7495-506.
Background Reference 4:
Haines BP and Rigby PW. Developmentally regulated expression of the LRRTM gene family during mid-gestation mouse embryogenesis. Gene Expr. Patterns 2007; 7:23-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6167

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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