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<strong>Figure 1 Western Blot Validation in Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: LSD1 3761 (0.2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: LSD1 3761 (0.2 μg/mL), LSD1 14-050 (1 μg/mL), beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse Tissues</strong><br>Loading: 15 μg of lysates per lane.Antibodies: LSD1 3761 (0.2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse P815 Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: LSD1 3761, (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunohistochemistry Validation of LSD1 in Human Breast Carcinoma Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human breast carcinoma Tissue using anti-LSD1 antibody (3761) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunofluorescence Validation of LSD1 in human breast carcinoma Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human breast carcinoma Tissue labeling LSD1 with 3761 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green/blue).
Figure 1 Western Blot Validation in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: LSD1 3761 (0.2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IAV
Trial Size

LSD1 Antibody

CATALOG NUMBER: 3761

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-LSD1 antibody (3761) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human LSD1.
The immunogen is located within amino acids 40-90 of LSD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 0.2 - 2 μg/mL; IHC-P: 2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1286 - P815 Cell Lysate
Positive Control 2:
Cat. No. 1224 - Daudi Cell Lysate
Positive Control 3:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 4:
Cat. No. 1211 – HepG2 Cell Lysate
Predicted Molecular Weight:
Predicted: 93kD
Observed: 110 kD
Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar LSD1 expression profile in human and mouse cell lines detected by two independent anti-LSD1 antibodies that recognize different epitopes, 3761 against N-terminus domain and 14-050 against recombinant fusion protein. LSD1 proteins are detected in the most tested cell lines that include mouse (3T3/NIH) and rat cell lines (YB2/0) at different expression levels by the two independent antibodies.

Isoforms:
Human LSD1 has 2 isoforms, including isoform 1 (852aa, 93kD) and isoform 2 (876aa, 95kD). Mouse LSD1 has one isoform (853aa, 93kD) and Rat LSD1 also has one isoform (872aa, 95kD). 3761 can detect all isoforms of human, mouse and rat.
Purification:
LSD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LSD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LSD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KDM1
Additional Names:
LSD1 Antibody: AOF2, KDM1, LSD1, BHC110, AOF2, KIAA0601, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110
Protein Accession Number:
NP_001009999
Protein Gi Number:
58761544
Ncbi Gene Id Number:
23028
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LSD1 Antibody: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.
Background Reference 1:
Shi et al. Cell 2004; 119:941-53.
Background Reference 2:
Kouzarides et al. Curr. Opin. Genet. Dev. 2002; 12:198-209.
Background Reference 3:
Shi et al. Mol. Cell 2005; 19:857-64.
Background Reference 4:
Nakamura et al. Mol. Cell 2002; 10:1119-28.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3761

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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