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<strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: LSD1 3763 ( 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: LSD1 3761 (0.2 μg/mL), LSD1 3763 (1 μg/mL), beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse P815 Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: LSD1 3763, (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of LSD1 in Human Small Intestine Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-LSD1 antibody (3763) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunofluorescence Validation of LSD1 in Human Small Intestine Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human small intestine cells labeling LSD1 with 3763 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Figure 1 Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: LSD1 3763 ( 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Green Product
IAV
Trial Size

LSD1 Antibody

CATALOG NUMBER: 3763

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-LSD1 antibody (3763) was raised against a peptide corresponding to 16 amino acids near the center of human LSD1.
The immunogen is located within amino acids 140-190 of LSD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 0.2 - 2 μg/mL; IHC-P: 2 μg/mL; IF: 20 μg/mL
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1286 - P815 Cell Lysate
Positive Control 2:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 3:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
Predicted: 93kD
Observed: 110 kD
Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar LSD1 expression profile in human and mouse cell lines detected by two independent anti-LSD1 antibodies that recognize different epitopes, 3761 against N-terminus domain and 3763 against central domain. LSD1 proteins are detected in the most tested cell lines that include mouse (3T3/NIH) and rat cell lines (YB2/0) at different expression levels by the two independent antibodies.

Isoforms:
Human LSD1 has 2 isoforms, including isoform 1 (852aa, 93kD) and isoform 2 (876aa, 95kD). Mouse LSD1 has one isoform (853aa, 93kD) and Rat LSD1 also has one isoform (872aa, 95kD). 3763 can detect all isoforms of human, mouse and rat.
Purification:
LSD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
LSD1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LSD1 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KDM1A
Additional Names:
LSD1 Antibody: AOF2, KDM1, LSD1, BHC110, AOF2, KIAA0601, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110
Protein Accession Number:
NP_001009999
Protein Gi Number:
58761544
Ncbi Gene Id Number:
23028
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LSD1 Antibody: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.
Background Reference 1:
Shi et al. Cell 2004; 119:941-53.
Background Reference 2:
Kouzarides et al. Curr. Opin. Genet. Dev. 2002; 12:198-209.
Background Reference 3:
Shi et al. Mol. Cell 2005; 19:857-64.
Background Reference 4:
Nakamura et al. Mol. Cell 2002; 10:1119-28.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3763

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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