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Western blot analysis using LSD1 monoclonal antibody against truncated LSD1 recombinant protein Hela cell lysate. and Jurkat cell lysate .
Immunohistochemical analysis of paraffin - embedded human lung carcinoma (top) and kidney carcinoma (bottom) showing nuclear localization using LSD1 antibody with DAB staining.
Western blot analysis using LSD1 monoclonal antibody against truncated LSD1 recombinant protein Hela cell lysate. and Jurkat cell lysate .

LSD1AOF2 Antibody [1B2F2 / 1B2E5]

CATALOG NUMBER: 32-188

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant human LSD1 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
LSD1/AOF2 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KDM1A
Additional Names:
AOF2, KDM1, KIAA0601, LSD1, BRAF35-HDAC complex protein BHC110AOF2, BHC110
Protein Accession Number:
O60341
Protein Gi Number:
51315808
Ncbi Gene Id Number:
23028
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The amine oxidase domain 2AOF2gene encodes a nuclear protein(LSD1,~95 kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serve as a docking module for the stabilization of the associated corepressor complex(es) on chromatin.
Background Reference 1:
Shi YJ, et.al Mol Cell. 2005 Sep 16;19(6):857-64.
Background Reference 2:
Metzger E, et.al Nature. 2005 Sep 15;437(7057):436-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-188

List Size:
0.1 mg

List Price:

$408.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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