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42-530 (0.05ug/ml) staining of Human Peripheral Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-530 (0.05ug/ml) staining of Human Liver lysate (lane 1), Mouse Liver lysate (lane 2) and Rat Liver lysate (lane 3) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-530 (0.05ug/ml) staining of Human Peripheral Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

LSP1 Antibody

CATALOG NUMBER: 42-530

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RYKFVATGHGKYEKV
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa band observed in Human Lymph Node, Tonsil and Peripheral Blood Mononucleocytes lysates (calculated MW of 37.2kDa according to NP_002330.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Exp Med. 2005 Feb 7;201(3):409-18; PMID: 15684321) . Recommended concentration: 0.05-0.2ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_002330.1; NP_001013271.1). Reported variants represent identical protein: NP_001013273.1, NP_001013271.1, NP_001013272.1
Positive Control 1:
Cat. No. 1315 - Human Tonsil Tissue Lysate
Positive Control 2:
Cat. No. 1369 - Human Lymph node Tissue Lysate
Positive Control 3:
Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
LSP1
Additional Names:
LSP1, lymphocyte-specific protein 1, WP34, pp52, 47 kDa actin binding protein, 52 kDa phosphoprotein, F-actin binding and cytoskeleton associated protein, leufactin (leukocyte F-actin binding protein), leukocyte-specific protein 1, lymphocyte-specific antigen WP34, lymphocyte-specific protein pp52
Protein Accession Number:
NP_002330.1,NP_001013271.1
Protein Gi Number:
10880979
Ncbi Gene Id Number:
4046

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-530

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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