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Western Blot at 1:8000 dilution Lane 1: Daudi whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
LSP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:8000 dilution Lane 1: Daudi whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.

LSP1 Antibody

CATALOG NUMBER: 61-932

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This LSP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-134 amino acids from the Central region of human LSP1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LSP1
Additional Names:
Lymphocyte-specific protein 1, 47 kDa actin-binding protein, 52 kDa phosphoprotein, pp52, Lymphocyte-specific antigen WP34, LSP1, WP34
Protein Accession Number:
P33241
Protein Gi Number:
462553
Ncbi Gene Id Number:
4046
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LSP1 is an intracellular F-actin bindingprotein. The protein is expressed in lymphocytes, neutrophils,macrophages, and endothelium and may regulate neutrophil motility,adhesion to fibrinogen matrix proteins, and transendothelialmigration. Alternative splicing results in multiple transcriptvariants encoding different isoforms.
Background Reference 1:
Davila, S., et al. Genes Immun. (2010)
Background Reference 2:
Odefrey, F., et al. Cancer Res. 70(4):1449-1458(2010)
Background Reference 3:
Bhatti, P., et al. Radiat. Res. 173(2):214-224(2010)
Background Reference 4:
Tamimi, R.M., et al. Cancer Causes Control (2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-932

List Size:
400 ul

List Price:

$495.00

Datasheet
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