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46-745 (0.05ug/ml) staining of Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-745 (5ug/ml) as the reporter antibody.
46-745 (2ug/ml) staining of paraffin embedded Human Blood Vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-745 (0.05ug/ml) staining of Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

LTF Antibody

CATALOG NUMBER: 46-745

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-ENYKSQQSSDPDP
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 85kDa band observed in Human Peripheral Mononucleocyte lysates (calculated MW of 78.2kDa according to NP_002334.2). Recommended concentration: 0.05-0.2ug/ml.Immunohistochemistry:In paraffin embedded blood vessel shows strong staining of cytoplasm of neutrophils.. Recommended concentration, 2-4ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant LTF captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
Positive Control 1:
Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
LTF
Additional Names:
LTF, lactotransferrin, GIG12, HLF2, LF, growth-inhibiting protein 12, lactoferrin, neutrophil lactoferrin, talalactoferrin
Protein Accession Number:
NP_002334.2
Protein Gi Number:
54607120
Ncbi Gene Id Number:
4057
Background Reference 1:
de la Rosa G, Yang D, Tewary P, Varadhachary A, Oppenheim JJ. Lactoferrin acts as an alarmin to promote the recruitment and activation of APCs and antigen-specific immune responses. J Immunol. 2008 May 15;180(10):6868-76.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-745

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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