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Western blot analysis of LUM antibody in A375 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with LUM Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of LUM antibody in A375 cell line lysates (35ug/lane)

LUM Antibody

CATALOG NUMBER: 63-035

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This LUM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the Central region of human LUM.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LUM
Additional Names:
Lumican, Keratan sulfate proteoglycan lumican, KSPG lumican, LUM, LDC, SLRR2D
Protein Accession Number:
P51884
Protein Gi Number:
20141464
Ncbi Gene Id Number:
4060
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LUM is a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, this protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. The protein is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair.
Background Reference 1:
Wang,P., Li,S. Invest. Ophthalmol. Vis. Sci. 50 (4), 1546-1551 (2009)
Background Reference 2:
Matsuda,Y., Yamamoto,T. Int. J. Oncol. 33 (6), 1177-1185 (2008)
Background Reference 3:
Kelemen,L.E., Couch,F.J. Breast Cancer Res. 10 (6), R98 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-035

List Size:
400 ul

List Price:

$495.00

Datasheet
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