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<b> Immunofluorescence staining of mouse splenocytes using anti-Ly6G/Ly6C antibody (RB6-8C5. </b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of RB6-8C5 (24-595) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-595- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.
<b> Flow-cytometry using the Anti-Ly6G/Ly6C antibody RB6-8C5 ( </b> Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r version of RB6-8C5 (24-595- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of mouse splenocytes using anti-Ly6G/Ly6C antibody (RB6-8C5. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of RB6-8C5 (24-595) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-595- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

Ly6G/Ly6C Antibody [RB6-8C5], Rabbit IgG

CATALOG NUMBER: 24-595

Clonality:
Recombinant Monoclonal
Tested Applications:
Depletion, Flow, IHC, WB
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Mouse
Species:
Rabbit
Original Species Name:
Rat
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)
Conjugate:
Unconjugated
Tested Applications:
Depletion, Flow, IHC, WB
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
Ly6g
Additional Names:
Gr-1, Gr1, Ly-6G, ly-6G.1, ly6C
Ncbi Gene Id Number:
546644
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-595

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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