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<b> Western Blot using anti-Lymphotoxin alpha antibody 3F12.2D3 (</b> Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the r-chimeric version of 3F12.2D3 (24-516) at 2 ug/ml before detection using an anti-rondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kDa- though the form shown here is likely glycosylated. 24-516 successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.
<b> Flow-cytometry using the anti-Lymphotoxin alpha antibody 3F12.2D3 (</b> U937 cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with unimmunized r antibody (MOPC-21; isotype control- black line) or the r-chimeric version of 3F12.2D3 (24-516- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Western Blot using anti-Lymphotoxin alpha antibody 3F12.2D3 ( Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the r-chimeric version of 3F12.2D3 (24-516) at 2 ug/ml before detection using an anti-rondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kDa- though the form shown here is likely glycosylated. 24-516 successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.

Lymphotoxin alpha Antibody [3F12.2D3], Rabbit IgG

CATALOG NUMBER: 24-516

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA, Flow, Neutralization Assay, Surface Plasma Resonance
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Mouse
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTα expressed in E. coli - the hybridoma clone was selected for its ability to bind LTα3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, Neutralization Assay, Surface Plasma Resonance
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
LTA
Additional Names:
LTα3 chimeric 3F12,LT,, LTα, Tumor necrosis factor beta, Tumor necrosis factor β, Tumor necrosis factor-beta, tumor necrosis factor-β, TNF beta, TNF-beta, TNF β, TNF-β, Lymphotoxin-alpha, Lymphotoxin alpha
Ncbi Gene Id Number:
4049
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-516

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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