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Western blot analysis of extracts of various cell lines, using Lyn antibody (14-007) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human stomach using Lyn antibody (14-007) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Lyn antibody (14-007) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using Lyn antibody (14-007) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.

Lyn Antibody

CATALOG NUMBER: 14-007

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
A synthetic peptide of human Lyn
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
A-549
Positive Control 2:
Mouse lung
Positive Control 3:
Mouse liver
Positive Control 4:
Mouse kidney
Predicted Molecular Weight:
Observed: 56kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Lyn
Additional Names:
LYN, v-yes-1 Yamaguchi sarcoma viral related oncogene homolog, FLJ26625, JTK8, Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog
Ncbi Gene Id Number:
4067
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-007

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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