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45-850 (0.1ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-850 (0.1ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

LYN Antibody

CATALOG NUMBER: 45-850

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-KTQPVRNTERT
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in lysates of cell line U937 (calculated MW of 58.6kDa according to NP_002341.1). Recommended concentration: 0.1-0.3ug/ml.
Positive Control 1:
Cat. No. 1215 - U937 Whole Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
LYN
Additional Names:
LYN , v-yes-1 Yamaguchi sarcoma viral related oncogene homolog , FLJ26625 , JTK8, Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog
Protein Accession Number:
NP_002341.1
Protein Gi Number:
4505055
Ncbi Gene Id Number:
4067
Background Reference 1:
Okamoto M, Hayakawa F, Miyata Y, Watamoto K, Emi N, Abe A, Kiyoi H, Towatari M, Naoe T. Lyn is an important component of the signal transduction pathway specific to FLT3/ITD and can be a therapeutic target in the treatment of AML with FLT3/ITD. Leukemia. 2007 Mar;21(3):403-10. Epub 2007 Jan 18.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-850

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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