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Western blot analysis of extracts of various cell lines, using LYZ antibody (15-033) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using LYZ antibody (15-033) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using LYZ antibody (15-033) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using LYZ antibody (15-033) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using LYZ antibody (15-033) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using LYZ antibody (15-033) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

LYZ Antibody

CATALOG NUMBER: 15-033

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LYZ (NP_000230.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HL-60
Positive Control 2:
U-937
Positive Control 3:
Mouse lung
Predicted Molecular Weight:
Observed: 13kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LYZ
Additional Names:
Lysozyme C,4-beta-N-acetylmuramidase C, LYZ, LZM
Ncbi Gene Id Number:
4069
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-033

List Size:
100 uL

List Price:

$436.00

Datasheet
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