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Western Blot at 1:8000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of CDH15 using rabbit polyclonal CDH15 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH15 gene (Lane 2).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with CDH15 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:8000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

M Cadherin Antibody

CATALOG NUMBER: 57-674

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This M Cadherin (CDH15) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 739-767 amino acids from the C-terminal region of human M Cadherin (CDH15).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
89 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDH15
Additional Names:
Cadherin-15, Cadherin-14, Muscle cadherin, M-cadherin, CDH15, CDH14, CDH3
Protein Accession Number:
P55291
Protein Gi Number:
1705553
Ncbi Gene Id Number:
1013
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDH15 is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. This protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation.
Background Reference 1:
Kang,J.S., Proc. Natl. Acad. Sci. U.S.A. 100 (7), 3989-3994 (2003) Hollnagel,A., Mol. Cell. Biol. 22 (13), 4760-4770 (2002) Meigs,T.E., Proc. Natl. Acad. Sci. U.S.A. 98 (2), 519-524 (2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-674

List Size:
400 ul

List Price:

$495.00

Datasheet
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