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To detect hM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human M-CSF (XP-5227) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hM-CSF.
To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human M-CSF (XP-5227) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hM-CSF.

M-CSF Antibody (biotin)

CATALOG NUMBER: XP-5227Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hM-CSF (human Granulocyte Colony Stimulating Factor).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect hM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human M-CSF (XP-5227) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hM-CSF.
Western Blot:
To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hM-CSF specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
M-CSF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CSF1
Additional Names:
MCSF, CSF-1Macrophage colony-stimulating factor 1, Lanimostim, CSF-1
Protein Accession Number:
P09603
Protein Gi Number:
311033367
Ncbi Gene Id Number:
1435
User Note:
Centrifuge vial prior to opening.
Background:
Four distinct colony-stimulating factors (CSFs) that promote survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte macrophage CSF (GMCSF), granulocyte CSF (GCSF), macrophage CSF (MCSF), and Interleukin-3 (IL-3, Multi CSF). Both GMCSF and IL-3 are multipotential growth factors, stimulating proliferation of progenitor cells from more than one hematopoietic lineage. In contrast, GCSF and MCSF are lineage restricted hematopoietic growth factors, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5227Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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